Effect of water extracts from Artemisia absinthium L. on feeding of selected pests and their response to the odor of this plant
The aim of this study was to determine the effect of water extracts from fresh and dry matter of Artemisia absinthium L. on the feeding of selected pests. Moreover their reactions on the smell of this plant by using the olfactometer were examined. Beetles feeding intensity assessment was carried out by measuring the surface of feeds caused by Sitona lineatus L. In the case of Acyrthosiphon pisum Harris contact poisoning on mortality of adults and larvae were tested. In determining the effect of extracts of Artemisia absinthium L. on Leptinotarsa decemlineata Say. food weight eaten by adult beetles and larvae and changes in larvae body weight were established. In studies on the olfactory reaction of abovementioned insects, for S. lineatus and L. decemlineata glass olfactometer “Y-tube” was used and for winged A. pisum females – 4-armed arena olfactometer. The results of the experiment showed that extract from dry matter of A. absinthium L. at concentration of 10% greatly reduced feeding of Sitona lineatus L. The highest mortality of A. pisum Harris was observed in objects in which extracts from dry and fresh matter of the highest concentrations (10% and 30% respectivelly) were applied. Extract from fresh matter in concentration of 30% made the greatest contribution to reduce feeding of L. decemlineata Say, while extract made from the dry matter at concentration of 10% significantly contributed to reduce the weight of food eaten by the larvae of this pest. Studies using the olfactometer showed a strong deterring reaction of odors derived from A. absinthium L. in relation to S. lineatus (both males and females) and males of L. decemlineata Say.
Keywords: Artemisia absinthium L., biotechnical methods, water extracts
Antidepressant and antioxidant activities of Artemisia absinthium L. at flowering stage
Artemisia absinthium (Asteraceae) is widely used in Iranian traditional medicine. Its effects may be correlated with the presence of antioxidant compounds. Methanolic extract of A. absinthium aerial part at flowering stage was screened for antioxidant activities by five complementary test systems. Also, its antidepressant activity was determined by forced swimming (FST) and tail suspension tests (TST). The extract showed good antioxidant activity. Also, the extract showed good reducing power activity between 50 and 800 µgml -1 . The extract exhibited a good activity in H2O2 scavenging (IC50 = 243 ± 12.15 µg ml -1 ). IC50 for iron ion chelating activity was 419 ± 20.95 µg ml -1 . Quercetin, BHA, EDTA and ascorbic acid used as positive controls in parallel experiments. The extract showed high phenolic and flavonoid contents. Extract showed good antidepressant activity in FST. The extract shortened remarkably the immobility period during the FST and TST and exhibited a dose dependent activity. All test groups were significantly different form control group (P < 0.001). Extract at 500 mg kg -1 showed similar activity as imipramine 10 mg kg -1 (p > 0.05) in TST. LD50 was 3700 mg/kg. These results introduced A. absinthium aerial parts as an easily accessible and edible source of natural antioxidants and antidepressant.
Key words: Antidepressant, Antioxidant activity, Artemisia absinthium, DPPH, forced swimming test, tail suspension test
More information: https://www.ajol.info/index.php/ajb/article/viewFile/68818/56885