Central nervous system activity of the hydroalcoholic extract of Casimiroa edulis in rats and mice
In order to evaluate the effects produced by the hydroalcoholic extract of leaves from Casimiroa edulis on the central nervous system, different behavioral tests and animal models of depression and anxiety were performed. The extract was administered intraperitoneally in male and female rats and tested on spontaneous motor activity, locomotor activity, exploration of an elevated plus-maze (EPM) and in the forced swimming test (FST). In addition, the extract was administered orally in male and female mice and evaluated in the following tests: general observation, pentobarbital-induced hypnosis, EPM, rota-rod, hole-board, and marble-burying. The results revealed that, in rats, the extract caused considerable reduction of locomotor and exploratory activities and increased the exploration of the EPM open arms in a similar way that diazepam. In the FST, the extract was as effective as fluoxetine in inducing shortening of immobility, along with a significant increase on climbing duration. On the other hand, in mice, the extract prolonged pentobarbital-induced hypnosis, increased exploration of the EPM open arms and partially protected from the pentylenetetrazol-induced convulsions. No significant effect was evident on motor coordination, hole-board and marble-burying tests. These results suggest that the hydroalcoholic extract of Casimiroa edulis may contain sedative principles with potential anxiolytic and antidepressant properties, which need further investigation.
Genetic Diversity of White Sapote (Casimiroa edulis La Llave & Lex.) Demonstrated by Intersimple Sequence Repeat Analysis
Phylogenic relationships among 31 cultivars of white sapote (Casimiroa edulis La Llave & Lex.) were examined by intersimple sequence repeat (ISSR) analysis. Polymorphic fragments were obtained in 24 of 100 ISSR primers (UBC Set#9, 801–900). Although ‘Cuccio’ and ‘Florida’ showed identical fragment patterns, the remaining cultivars could be distinguished from each other. The present study demonstrated the usefulness of ISSR analysis for cultivar identification and phylogenic study in white sapote.
More information: http://hortsci.ashspublications.org/content/42/6/1329.full.pdf