Anti-inflammatory Activity of Methanolic Extract of Hibiscus sabdariffa on Carrageenan Induced Inflammation in Wistar Rat
Background: Hibiscus sabdariffa is used for medicinal purposes, especially in alternative medicine. It is a folk remedy for abscesses, cancer, cough, debility, dyspepsia, dysuria, fever, hangover, heart ailments, neurosis, scurvy and strangury. This present study was carried out to investigate the anti-inflammatory activity of methanolic extract of hibiscus sabdariffa in adult wistar rat.
Methods: A total of Twenty-five (25) of both sexes rats weighing between 180 – 200 g were randomly divided into five (5) groups of five (5) animals each. Group 1 received only 1 ml distilled water which served as control. Group 2 were induced with inflammation using (0.1ml/kg b.w) carrageenan without treatment while Group 3 and 4 were induce with inflammation and treated with low dose (250 mg/kg b.w) and high dose (500 mg/kg b.w) of extract of hibiscus sabdariffa respectively. Group 5 served as the positive control group, they were treated with (20mg/kg b.w) diclofenac after carrageenan induced inflammation. The paw diameter of each rat was measured before and after the inflammation then after treatment.
Results: There was significant reduction (p< 0.05) in paw diameter in the group that received high dose (500 mg/kg b.w) of methanolic extract of hibiscus sabdariffa from Mean±SEM of 0.566±0.023 to 0.414±0.009 as compared with the untreated group.
Conclusions: The decrease in paw diameter in the high dose group showed that methanolic extract of hibiscus sabdariffa possess anti-inflammatory properties against carrageenan induced inflammation similar to the action of diclofenac. The therapeutic effect of this extract will encourage its use in the treatment of inflammation.
Keywords: Hibiscus sabdariffa, anti-inflammatory, carrageenan, diclofenac, paw diameter.
More information: http://www.ijpsi.org/Papers/Vol2(3)/Version-2/E232224.pdf
Monitoring The Antibacterial Activity Of Hibiscus Sabdariffa Extracts
ABSTRACT This paper focuses on studying the antibacterial effect of Hibiscus sabdariffa extracts, a plant known in traditional medicine for its beneficial effects on the body. Hibiscus sabdariffa extracts were done in pure water, 50% ethyl alcohol, and 96% ethyl alcohol; the concentrations used to determine antibacterial activity were 1:1, 1:5, 1:10, 1:25, 1:50, noted cu 1, 2, 3, 4, 5. The antibacterial effect of the extracts was tested using the impregnated disk-diffusion method on bacteria Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Enterobacter clocae ATCC 13047, Klepsiella pneumoniae ATCC 13833, Staphylococcus aureus ATCC 29213, Yersinia enterocolitica ATCC 9610. We ascertained that the most efficient extracts were those obtained using 96% ethyl alcohol, 1:1 concentration on Escherichia coli ATCC 25922 and Enterobacter clocae ATCC 13047 strains, where the inhibitory action presented average values between 5.43 mm and 3.43 mm. In the case of Klepsiella pneumoniae ATCC 13833 and Yersinia enterocolitica ATCC 9610 strains, the extracts recorded minimum or zero values. The antibacterial activity of Hibiscus sabdariffa depended on their concentration, on the solvent used, as well as on the resistance of the bacteria under study to the bioactive compounds in them.
Keywords: Hibiscus sabdariffa extracts, bacterial strains, antibacterial activity